The fatigue state of nuclear power operators is of vital importance to the safe operation of a nuclear power plant. With the development of the digital manipulation interface in recent years, the fatigue type and degree of operators have undergone changes. The interface of the nuclear power monitoring task is simulated in this experiment, and multiple fatigue detection modes are used to measure the fatigue state of operators. Results show that the subjective fatigue reporting method and PERCLOS P80 can reflect the change of fatigue state. The correlation between task performance and fatigue is not remarkable, and the sensitivity of the electrocardiographic heart rate variability indicator is poor. The accuracy also remains to be further verified.