The total neutron cross section of 58Fe has been determined in the energy range from 7 to 325 keV by a transmission measurement using samples enriched in the 58Fe isotope. The data have been shape-fitted by means of an R matrix multilevel formalism to extract resonance parameters for s and l > 0 wave resonances. The s-wave strength function was determined to S0 = (4.3 ± 1.9) × 10-4.