The production behavior of irradiation defects in lithium oxide was studied by in-situ luminescence measurement under He+ beam irradiation. The luminescence peaks of 380 nm and 340 nm, which were associated with the production of F+ and F0 centers, respectively, were measured under various conditions such as temperature changes, and the production mechanism and kinetics of these irradiation defects were determined. The F+ centers are rather directly produced from Li2O with the partners of O- interstitials under Coulomb interactions while the F0 centers are produced with O2 through some diffusion processes. Although the F+ production dominates at lower temperatures, the F0 production increases with increasing temperature. The F0 centers would play an important role in the tritium recovery from ceramic breeder materials at actual blanket conditions.